LOCUS       40924_949        9281 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GAANTRY B donor MoroMYB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Collier R, Thomson JG, Thilmony R.
  TITLE     GAANTRY, a versatile and robust Agrobacterium-based gene stacking 
            system generates high quality transgenic plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2017) Crop Improvement and Genetics research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 9281)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9281)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-DEC-2017) Crop Improvement and Genetics research Unit, 
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(7..218)
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     CDS             complement(join(232..772,1568..1697,1795..1912))
                     /codon_start=1
                     /product="Moro MYBA"
                     /label=Moro MYBA
                     /note="derived from Citrus sinensis"
                     /protein_id="AWK49560.1"
                     /translation="MADSLGVRKGAWTGEEDDLLRKCIEKYGEAKWHQVPLRAGLHRCR
                     KSCRLRWLNYLNPNIKRGEFAADEVDLILRLHKLLGNRWSLIVGRLPGRTANDVKNFWN
                     THLRKKVDKCCKNNKEMKAKAEKVEKINIIKPQPRTFAKNSQWLKGKGMTSNNLQLGDY
                     NLGKQSTPSDHHHHHQQQQENETESVWWESFLFGDELDQQGISSSLSRPEEESTTANIF
                     AEKSPVVTKVTENRVIEAGQSCPTDDFAFDAELWDLLNAK"
     regulatory      complement(1924..3163)
                     /label=Arabidopsis HotHead (HTH) promoter
                     /note="Arabidopsis HotHead (HTH) promoter"
                     /regulatory_class="promoter"
     misc_feature    3224..3278
                     /label=TP901 attB
                     /note="TP901 attB"
     misc_feature    3300..3324
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        3483..3511
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    3552..3657
                     /label=ParA MRS
                     /note="ParA MRS"
     CDS             3700..4230
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     misc_feature    4309..4414
                     /label=ParA MRS
                     /note="ParA MRS"
     primer_bind     complement(4437..4453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4461..4477)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4485..4515)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4530..4551)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4784..5229
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             5230..6648
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     rep_origin      complement(6749..7337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7511..8368)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8369..8473)
                     /label=AmpR promoter
     rep_origin      complement(8499..8954)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     9096..9112
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cctgcaggtc actggatttt ggttttagga attagaaatt ttattgatag aagtatttta
       61 caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga aaccctataa
      121 gaaccctaat tcccttatct gggaactact cacacattat tctggagaaa aatagagaga
      181 gatagatttg tagagagaga ctggtgattt ttgcggactc tagaactagt cttactttgc
      241 attgagaaga tcccaaagtt ccgcgtcgaa agcgaagtca tcagtcggac aactctggcc
      301 tgcttcaatg actctatttt ctgtcacctt tgtcactact ggagactttt cggcaaaaat
      361 atttgccgta gtagattcct cttctggccg actcaatgag cttgaaattc cttgttgatc
      421 caattcatct ccgaataaaa aactttccca ccatacagat tcagtttcat tctcctgctg
      481 ctgttgatga tgatgatgat gatcagacgg ggtggattgt ttgccgagat tgtaatctcc
      541 taattgcaaa ttatttgaag tcattccctt gcccttcaac cattgtgagt ttttagcgaa
      601 ggtccgaggt tgaggtttta tgatattgat cttttccacc ttctcagctt ttgctttcat
      661 ctctttatta ttcttgcagc atttatccac tttcttgcgc aggtgcgtgt tccaaaaatt
      721 cttcacatcg ttcgctgttc ttcccggaag cctgcccaca atcagtgacc atctacatgc
      781 aattcaacca aattacagta tatcagaatt gcattttgcc aacaaaaaag ggacaaaacg
      841 ataatttata ataaaagtaa agttgaaccc acatcctgtt acaaattctg tgtcataatt
      901 ttgtaagttt agtactactt aaatatatat tctgcagctt taaaagggtg gccgagaggt
      961 tggataacca tccatattcg aataataaga aattaaagaa tagaaattaa taattggata
     1021 accatccatc gtcgtgttat tgtaaaaggc tgaatttgaa aggtctaaca tgtcaccatt
     1081 ccctgaaaag tacatcataa atttctgatt aattcctatt taattccggc ctacaacctg
     1141 taaccctatg tacatgccac gaaaagaaaa atctgctaat atagtaagag ttctaatttg
     1201 cgaaaacttt gtgacaagtg ttttgttaga tgatggggta gtttatgtgt atgctatatg
     1261 ttgctcaacc aatatgcaca cgtagctatt ggaccaccct tttagtttct ctttactcca
     1321 attagtatat tttcctatag tgttcgatct acatgattaa aatttaatgt taatcatata
     1381 tttggacaag ataataaaag gaatataggt gtctcatcaa attaatgtgg agtaaaaata
     1441 actatgatta actcccaaat aatagttata agtgtttgtt gaaatggctt aattaaaagt
     1501 caccgctggt agacggtaat actaaacgga gcctcagttc ggcttccgtt ctactttctg
     1561 cacttgcctg ttgcccagca gcttatggag ccttaaaatt aaatcaactt catctgctgc
     1621 aaattctcct cgtttgatat tcgggttgag atagttcagc caccgcagtc tacagctttt
     1681 ccggcatcga tgcaatcctg aaattgttca aaagagagaa tataaactgt tgaaattgca
     1741 taaatagaag cagctcctgt gaaaactact tgaaatagta ctgaaactat ttacctgctc
     1801 ttagaggaac ttgatgccat tttgcttccc catatttctc aatgcatttc ctaagaagat
     1861 catcttcctc tcctgtccat gcacctttac gaactcctaa ggaatccgcc atgaattctc
     1921 gagtggagac tgcagaaacc aaaactgaag agcttttttt tttaatgttt ttttgttttg
     1981 ttattattgc ttctgcatca acttcttatg tggcttttga tgaatggtct tgttttgtgt
     2041 gttttcttct cctataaata acactaacga tccaacagag caactatatt aattacaccc
     2101 acatgtgtct ctatatattg ttacatatat gtaaccattt ctacgagttg tataacttat
     2161 agtcttatac ggtacattga attttggtta gacaacaaca ttcggtaaaa aaatcatata
     2221 gtaaaaaaaa acacaagttg tgtaataaag agatgttggg gtggtctaca ctaaaacgta
     2281 aatttgattt taggtgtgtc ccattctgtc gaatatagca aatgaaaagg acatatgaat
     2341 catcttactc aattaatagt tccctcattt actcataaaa aatagttacc tactaattta
     2401 ttttaccagc tagtaacttt cctcacatta gacttattgt tacagaattg tttgttgcaa
     2461 attgaaatta atgataagta cttcgttaca aagaacaatc actatcaaat aacatctttg
     2521 tatttatatt gaaactaagc cttaaaagga tttcctttca cattttttta ccttggtgat
     2581 gataataaga ataataactc gtttttttgt cccatgattt tgacttttgg gaccagctgc
     2641 aaagaaaaac aatttgttag tacttagtaa tagtattact gtaaaatcat ataaaacaat
     2701 aaagagataa aaaggatagt agtacagctt gtctgtatca ttaatggcga tatgaaaaca
     2761 gttgaagtcg gagatctggt agccgttgga tcaagtcttc atagattcat agtgtatcaa
     2821 ttaatcatct tcacctaacc aaaatctaaa aactatacta tacagagcca tgtttttggc
     2881 aagtcattta attactatta gtcataaact tattaattat gactgtaggt tggttactaa
     2941 cataaatctt cacgggttat gtttttggca actaatttaa ttgttctttc acctaaccaa
     3001 aaacaatatc atccgtttta tttggttgag cgtgaatgtg atcatcagtc agtacttagt
     3061 actaacaact aacaaaacat attggattag ttgccaaatg taatacaaaa acaatcaagg
     3121 ttttaacttt agtgttgaat gaaaaatgtc acatcgcatc acgcacgtgg actgacctgc
     3181 aggaaaggta ccaaaccatg gaaaggcgcg ccgtttaaac aaactgataa ttgccaacac
     3241 aattaacatc tcaatcaagg taaatgcttt ttgcttttaa agtttaaact atcagtgttt
     3301 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     3361 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     3421 ggttcccctc gggagaaacg tacggacgca caccgtggaa acggatgaag gcacgaaccc
     3481 agttgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg cgctcacgca
     3541 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     3601 cttgttatga ctgttttttt gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     3661 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagcaacgat
     3721 gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat gggcatcatt
     3781 cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct tgatcttttc
     3841 ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc cgattacctc
     3901 gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca agaagcggtt
     3961 gttggcgctc tcgcggctta cgttctgccc aagtttgagc agccgcgtag tgagatctat
     4021 atctatgatc tcgcagtctc cggagagcac cggaggcagg gcattgccac cgcgctcatc
     4081 aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt gcaagcagat
     4141 tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga agaagtgatg
     4201 cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga gatcggcttc
     4261 ccggccgcgg agttgttcgg taaattgtca caacaccgcc gtacgaaagg tcaaattggg
     4321 tatacccatt tgggcctagt ctagccggca tggcgcatta cagcaatacg caatttaaat
     4381 gcgcctagcg cattttcccg accttaatgc gcctaaacgc gcttggcgta atcatggtca
     4441 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4501 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4561 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     4621 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4681 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4741 cggttatcca cagaatcagg ggataacgca ggaaagaaca tggcacatat acctgccgtt
     4801 cactattatt tagtgaaatg agatattatg atattttctg aattgtgatt aaaaaggcaa
     4861 ctttatgccc atgcaacaga aactataaaa aatacagaga atgaaaagaa acagatagat
     4921 tttttagttc tttaggcccg tagtctgcaa atccttttat gattttctat caaacaaaag
     4981 aggaaaatag accagttgca atccaaacga gagtctaata gaatgaggtc gaaaagtaaa
     5041 tcgcgcgggt ttgttactga taaagcaggc aagacctaaa atgtgtaaag ggcaaagtgt
     5101 atactttggc gtcacccctt acatatttta ggtctttttt tattgtgcgt aactaacttg
     5161 ccatcttcaa acaggagggc tggaagaagc agaccgctaa cacagtacat aaaaaaggag
     5221 acatgaacga tgaacatcaa aaagtttgca aaacaagcaa cagtattaac ctttactacc
     5281 gcactgctgg caggaggcgc aactcaagcg tttgcgaaag aaacgaacca aaagccatat
     5341 aaggaaacat acggcatttc ccatattaca cgccatgata tgctgcaaat ccctgaacag
     5401 caaaaaaatg aaaaatatca agttcctgag ttcgattcgt ccacaattaa aaatatctct
     5461 tctgcaaaag gtctggacgt ttgggacagc tggccattac aaaacgctga cggcactgtc
     5521 gcaaactatc acggctacca catcgtcttt gcattagccg gagatcctaa aaatgcggat
     5581 gacacctcga tttatatgtt ctatcaaaaa gtcggcgaaa cttctattga cagctggaaa
     5641 aacgctggcc gcgtctttaa agacagcgac aaattcgatg caaatgattc tatcctaaaa
     5701 gaccaaacac aagaatggtc aggttcagcc acatttacat ctgacggaaa aatccgttta
     5761 ttctacactg atttctccgg taaacattac ggcaaacaaa cactgacaac tgcacaagtc
     5821 aacgtatcag catcagacag ctctttgaac atcaacggtg tagaggacta taaatcaatc
     5881 tttgacggtg acggaaaaac gtatcaaaat gtccagcagt tcatcgacga aggcaactac
     5941 agctcaggcg acaaccatac gctgagagat cctcactacg tagaagataa aggccacaaa
     6001 tacttagtat ttgaagcaaa cactggaact gaagatggct accaaggcga agaatcttta
     6061 tttaacaaag catactatgg caaaagcaca tcattcttcc gtcaagaaag tcaaaaactt
     6121 ctgcaaagcg ataaaaaacg cacggctgag ttagcaaacg gcgctctcgg tatgattgag
     6181 ctaaacgatg attacacact gaaaaaagtg atgaaaccgc tgattgcatc taacacagta
     6241 acagatgaaa ttgaacgcgc gaacgtcttt aaaatgaacg gcaaatggta tctgttcact
     6301 gactcccgcg gatcaaaaat gacgattgac ggcattacgt ctaacgatat ttacatgctt
     6361 ggttatgttt ctaattcttt aactggccca tacaagccgc tgaacaaaac tggccttgtg
     6421 ttaaaaatgg atcttgatcc taacgatgta acctttactt actcacactt cgctgtacct
     6481 caagcgaaag gaaacaatgt cgtgattaca agctatatga caaacagagg attctacgca
     6541 gacaaacaat caacgtttgc gccgagcttc ctgctgaaca tcaaaggcaa gaaaacatct
     6601 gttgtcaaag acagcatcct tgaacaagga caattaacag tcaacaaata aaaacgcaaa
     6661 agaaaatgcc gatatgctat cggcattaca tgtgagcaaa aggccagcaa aaggccagga
     6721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6901 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6961 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7021 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7081 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7141 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7201 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7261 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7321 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7381 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7441 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7501 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7561 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7621 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7681 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7741 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7801 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7861 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7921 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7981 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8041 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8101 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8161 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8221 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8281 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8341 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8401 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8461 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     8521 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     8581 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     8641 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     8701 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     8761 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     8821 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     8881 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     8941 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     9001 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     9061 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgt
     9121 taacaaaagt acttttagat ctgtaacttt ttcggatcaa gctatgaagg acgcaaagag
     9181 ggaactaaac acttaattac gcgttttata acggtcctaa ggtagcgaaa aatagggata
     9241 acagggtaat catcctcgac aaaatcgata aagagctcaa a
//
